Mammalian Mitochondria Possess Homologous DNA Recombination Activity
نویسندگان
چکیده
منابع مشابه
Homologous recombination in mammalian cells.
Mouse LTKcells were transfected with a pair of defective Herpes simplex virus thymidine kinase ( t k ) genes. One tk gene had an 8-bp insertion mutation while the second gene had a 100-bp inversion. Extrachromosomal homologous recombination leading to the reconstruction of a functional tk gene was monitored by selecting for tk positive cells using medium supplemented with hypoxanthine/ aminopte...
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Mammalian mitochondrial protein extracts possess DNA end-binding (DEB) activity. Protein binding to a 394 bp double-stranded DNA molecule was measured using an electrophoretic mobility shift assay. Mitochondrial DEB activity was highly specific for linear DNA. Inclusion of a vast excess of non-radioactive circular DNA did not disrupt binding to radioactive f394. In contrast, binding was abolish...
متن کاملCharacterization of homologous DNA recombination activity in normal and immortal mammalian cells.
Homologous DNA recombination levels were measured in normal and spontaneously immortalized murine and human fibroblasts, and in a number of primate and murine established fibroblast cell lines. Immortal cell lines and tumor-derived clones homologously recombined extrachromosomal plasmid substrates at frequencies approximately 100-fold higher than did normal cells. To further explore the mechani...
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Gene targeting in mammalian cells has proven invaluable in biotechnology, in studies of gene structure and function, and in understanding chromosome dynamics. It also offers a potential tool for gene-therapeutic applications. Two limitations constrain the current technology: the low rate of homologous recombination in mammalian cells and the high rate of random (nontargeted) integration of the ...
متن کاملFunctions of homologous DNA recombination
Homologous DNA (or genetic) recombination plays important roles in heredity and evolution. A recent study has shown that a physical linkage established by recombination between two or more homologous DNA regions or molecules is essential for the precise disjunction of homologous chromosomes in meiosis and the recovery of collapsed DNA replication forks. These physical linkages in recombination ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1996
ISSN: 0021-9258
DOI: 10.1074/jbc.271.44.27536